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Using mRNA differential display we have identified differentially expressed genes in non-self-interacting vs. single mycelia of the conifer pathogen Heterobasidion annosum and the wood decomposing basidiomycete Physisporinus sanguinolentus. Altogether 39 differentially displayed bands were cloned and sequenced, corresponding to 21 unique genes, which were confirmed by semi-quantitative RT-PCR to be differentially expressed. Further confirmation of differential gene expression Barbour Belgique was made by real time RT-PCR. All 10 genes identified from P. sanguinolentus had lower expression, while in H. annosum three genes had higher and eight lower expression in non-self-interacting mycelia vs. single mycelia. One of the induced genes showed high similarity to the Coprinus cinereus recA/RAD51 homolog (rah1) which is essential for homologous recombination, DNA repair and Barbour Sale stress responses.
Magnetically diluted systems of dithiocarbamate complexes of the type 6365Cu⧹ [Ni (II) — Me (II) — Dtc] (Me = Zn, Cd, Hg ; Dtc = MDtc (dimethyl-) or EDtc (diethyldithiocarbamate) ) , which model the structural heterogeneity on the local, molecular and supramolecular levels, have been prepared and studied by means of ESR and solid state 13C and 15N CP⧹MAS NMR spectroscopy.
Three possible structures of these dithiocarbamate complexes were Acheter Veste Barbour Homme unambiguously identified in the composition of the samples under investigation : (i) the mononuclear square-planar, Ni (Dtc) 2 ; (ii) dinuclear with non-planar molecular configurations, Me2 (Dtc) 4 and (iii) heteropolynuclear complexes with a common composition of Me2(Dtc)4 · nNi (Dtc) 2 (n = 1–4 ; Me = Zn, Cd, Hg) . The latter is a newly proposed type of structure of dithiocarbamate complexes in the matrix of the model systems.
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